Actin-mediated Delivery of Astral Microtubules Instructs Kar9p Asymmetric Loading to the Bud-Ward Spindle Pole
نویسندگان
چکیده
منابع مشابه
Actin-mediated Delivery of Astral Microtubules Instructs Kar9p Asymmetric Loading to the Bud-Ward Spindle Pole
In Saccharomyces cerevisiae, Kar9p, one player in spindle alignment, guides the bud-ward spindle pole by linking astral microtubule plus ends to Myo2p-based transport along actin cables generated by the formins Bni1p and Bnr1p and the polarity determinant Bud6p. Initially, Kar9p labels both poles but progressively singles out the bud-ward pole. Here, we show that this polarization requires cell...
متن کاملCortical capture of microtubules and spindle polarity in budding yeast - where's the catch?
In asymmetric divisions, the mitotic spindle must align according to the cell polarity axis. This is achieved through targeting astral microtubules emanating from each spindle pole to opposite cell cortex compartments. Saccharomyces cerevisiae is a powerful genetic model for dissection of this complex process. Intense research in this yeast has rendered detailed models for a program linking act...
متن کاملMicrotubule Cytoskeleton: Navigating the Intracellular Landscape Dispatch
The intracellular cytoskeleton in embryos and single-cell eukaryotes is extremely dynamic and constantly remodeled during growth and differentiation. Microtubules and actin filaments comprise the filamentous cytoskeletal network. We are starting to understand how microtubules and actin filaments collaborate to ‘read’ intracellular cues. Microtubules are nucleated from microtubule organizing cen...
متن کاملDissecting the involvement of formins in Bud6p-mediated cortical capture of microtubules in S. cerevisiae.
In S. cerevisiae, spindle orientation is linked to the inheritance of the ;old' spindle pole by the bud. A player in this asymmetric commitment, Bud6p, promotes cortical capture of astral microtubules. Additionally, Bud6p stimulates actin cable formation though the formin Bni1p. A relationship with the second formin, Bnr1p, is unclear. Another player is Kar9p, a protein that guides microtubules...
متن کاملSpatial cues and not spindle pole maturation drive the asymmetry of astral microtubules between new and preexisting spindle poles
In many asymmetrically dividing cells, the microtubule-organizing centers (MTOCs; mammalian centrosome and yeast spindle pole body [SPB]) nucleate more astral microtubules on one of the two spindle poles than the other. This differential activity generally correlates with the age of MTOCs and contributes to orienting the mitotic spindle within the cell. The asymmetry might result from the two M...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 2010
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.e10-03-0197